Posts Tagged: sorghum
As sorghum plants cope with drought conditions, the plants' roots and adjoining microbial communities are communicating in a chemical language that appears to improve the plants' chances under water stress.
“It's amazing,” said Peggy Lemaux, UC Cooperative Extension specialist. “We know there are lots of microbes in the soil and, for the most part, ones in the surrounding soil stayed the same under drought conditions. We only saw changes in those microbes closely associated with the roots.”
The role of drought in restructuring the root microbiome was the first published discovery to come out of a sweeping drought research project underway since 2015 in the fields at UC Kearney Research and Extension Center in Parlier. The five-year study, funded with a $12.3 million grant from the Department of Energy, aims to tease out the genetics of drought tolerance in sorghum and its associated microbes. Using sorghum as a model, scientists hope the research will help them understand and improve drought tolerance in other crops as well.
The new research results from the lab of USDA's Devin Coleman-Derr at UC Berkeley, published April 16, 2018, in the Proceedings of the National Academy of Sciences, document the fate of microbes associated with sorghum roots under three distinct irrigation regimens. Because the San Joaquin Valley generally sees no rain during the growing season, it is the ideal place to mimic drought conditions by withholding irrigation water.
All plots received a pre-plant irrigation to initiate growth. In the control plots, sorghum was irrigated normally, with weekly watering through the season. In the plot simulating pre-flowering drought stress, the plants received no additional water until flowering, about halfway through the season. The third treatment was watered normally until it flowered, and then water was cut off for the rest of the season.
Beginning when the plants emerged, the scientists collected samples from each plot on the same day and time each week for 17 weeks. In a mini, in-field laboratory, roots, rhizosphere (zone surrounding the root), leaves and soil samples from 10 plants in each plot were immediately frozen and transported to Berkeley, where they were disseminated to collaborators, who investigated the plant and microbial responses at the molecular level.
“When a sorghum plant is subjected to drought, it starts sloughing off metabolites, nutrients and amino acids from the roots. The compounds appear to communicate to the neighboring microbial community that the plant is under stress,” Lemaux said. “That selects out a certain population of microbes. Certain types of microbes increase, others go away. When you add water back, the microbial community returns to its pre-drought population in just a few days.”
The researchers cultured two specific microbes that were enriched in the rootzone under drought conditions. They coated sorghum seeds with the microbes and planted them under drought conditions in a growth chamber. This treatment encouraged the plant to grow more roots.
“The microbes appear to improve plant growth during drought,” Lemaux said. “Those microbes appear to be helping plants survive drought. We didn't know that was happening before we got these results.”
Lemaux said the research might lead to future field use of the research breakthrough.
“A lot of companies are interested in the microbiome,” she said. “Some are already selling microbes to coat seeds.”
A team of researchers has received a $5 million grant from the U.S. Department of Agriculture to find new ways to combat Johnsongrass, one of the most widespread and troublesome agricultural weeds in the world.
“Johnsongrass is a huge problem,” said Jeff Dahlberg, UC Cooperative Extension sorghum specialist and director of the UC Kearney Agricultural Research and Extension Center in Parlier, Calif. “It impacts many different crops and is very hard to control.”
Dahlberg is part of the team that includes scientists from Virginia, Kansas, North Carolina, Texas and Georgia. Andrew Paterson, director of the Plant Genome Mapping Laboratory at the University of Georgia, Athens, is the lead investigator.
The naturalization of Johnsongrass across much of the U.S. has also allowed the plant to develop attributes — such as cold and drought tolerance, resistance to pathogens and the ability to flourish in low-fertility soils — that make it particularly difficult to control. Adding to the challenge is the adoption of herbicide-resistant crops around the world.
“Herbicide-resistant crops have been associated with a dramatic increase in herbicide-resistant weeds,” Patterson said. “With 21 genetically similar but different types of Johnsongrass known to be resistant to herbicides, it will only become more problematic in the future.”
Over the course of their five-year project, the researchers will work to better understand the weed's capabilities and the genes that make Johnsongrass so resilient. Johnsongrass [Sorghum halepense] is closely related to sorghum [Sorghum bicolor (L.) Moench], a healthy gluten-free grain, animal feed and biofuel crop. Lessons learned from the Johnsongrass research may lead to strategies to improve sorghum.
For his part, Dahlberg plans to use the global information system (GIS) to map the locations of Johnsongrass in California to better record its distribution in the state and to help understand how it spread into California by relating it to other populations of johnsongrass in the U.S.
“Ideally, we will use an app to map, identify, manage, and catalog populations that have developed different traits – such as susceptibility to plant disease, ability to host a particular insect, or resistance to herbicides,” he said.
This information may lead to new management strategies that target and curb its growth, providing farmers with more options to combat the invasive plant. The researchers also hope that learning more about the fundamental structures that give Johnsongrass its unusual resilience will pave the way for new genetic tools to improve useful plants, such as sorghum.
Other researchers working on this project are Jacob Barney, Virginia Tech; C. Michael Smith, Kansas State University; Wesley Everman, North Carolina State University; Marnie Rout, University of Texas, Temple; and Clint Magill and Gary Odvody, Texas A&M University.
California’s role as an emerging world leader in the development of green energy technologies offers the state’s farmers the opportunity to diversify their cropping systems and increase their income.
Sacramento lawmakers have given the California Energy Commission an annual budget of $100 million to support the development of alternative and renewable low-carbon fuels. In addition, the State Alternative Fuels Plan set goals of reducing petroleum dependence by 15 percent and increasing alternative fuels use by 20 percent by 2020. These efforts are meant to help meet the growing fuel demands of the world population while reducing greenhouse gas emissions in California to 1990 levels.
“With the new mandates, there are new opportunities for using agricultural waste and dedicated energy crops for biofuels, but we’re not yet sure exactly what form it will take,” said UC Cooperative Extension alfalfa specialist Dan Putnam. “I would always encourage growers to experiment with the crop, but I wouldn’t jump in whole hog unless I had a buyer lined up.”
U.S. ethanol production in January 2010 was 818,000 barrels per day, according to the U.S. Energy Information Administration. The United States uses about 20 million barrels of oil per day. If the EPA allows up to 15 percent ethanol blends for all vehicles, then the 10 million barrels per day of oil used by cars and trucks could allow ethanol and biofuels to make up 1.5 million barrels per day.
Cellulosic biomass is the only known resource for the sustainable production of liquid transportation fuels on a large scale and at low costs, according to UC Riverside environmental engineer C. W. Wyman. Cellulosic biomass includes agricultural residues such as corn stems and leaves, forestry residues such as sawdust and paper, landscape waste, herbaceous plants such as switchgrass and sorghum, and woody plants such as poplar trees.
Since a dry ton of cellulosic biomass could provide about three times as much energy as a barrel of petroleum, the cellulosic biomass would have three times the value as a barrel of petroleum. That means cellulosic biomass would be worth about $200 per dry ton when crude oil sells at $65 per barrel.
“To utilize this abundant resource, we must develop low-cost technologies for transforming biomass into fuels that can compete with petroleum,” Wyman wrote in a California Agriculture article “Cellulosic biomass could meet California’s transportation fuel needs.”
Current and potential biomass crops include the grasses switchgrass and miscanthus, other perennial grasses, plus high biomass sorghum, alfalfa and other crops.
Across California, University of California scientists are studying potential biofuel crops. Putnam has four research trials under way, testing varieties from Ceres and Mendel Biotechnology, Inc. He said switchgrass and miscanthus the top contenders so far in his trials.
“The yields of switchgrass under irrigation have been quite high,” Putnam said. “It is an efficient crop for converting solar energy into biomass under warm weather condition.”
Switchgrass does have relatively high water needs. Putname said scientists are looking into whether the crop can be grown under deficit irrigation to save water and still produce the biomass.
“The key issue with biofuels is not necessarily the total water requirement, but the water use efficiency and amount of biomass produced per unit of water,” Putnam said. “Even if a crop has high water use, if it produces a large amount of biomass, it may still be the best option.”
Steve Kaffka and UC Cooperative Extension advisors are involved in research on winter annual oilseeds such as canola, camelina and meadowfoam as potential biofuel feedstock crops.
“Recent economic modeling we have done suggests that at current market prices, canola is a competitive crop in California, but outlets have not yet developed for the seed,” Kaffka said. “Currently, petroleum prices are too low to support the use of canola for biofuel feedstocks, but that is changing rapidly. “
The director of the UC Kearney Research and Extension Center, Jeff Dahlberg, sees opportunities for California production of sorghum as a biofuel crop.
"Sorghum is one of the few crops that span all the different renewable fuel options," he said. "You can use the grain to convert into ethanol. We have sweet sorghum, a specialty sorghum which is very similar to sugar cane. You can press the juice out and convert it into ethanol. And, we can produce a lot of biomass."
Dahlberg previously served as research director for the National Sorghum Producers and the research director for the United Sorghum Checkoff Program in Lubbock, Tex. He currently is lead investigator on a $984,000 U.S. Department of Energy grant to study the composition of sorghum and its potential for cellulosic conversion to biofuel. In addition to continuing this research at Kearney, Dahlberg is interested in developing a center for on-farm green technologies at the Central California research station.